Manli Weng

Chinese Academy of Sciences

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Baotai Guo

Qingdao Agricultural University

Jianwei Sun

Yunnan University

Delin Duan

Institute of Oceanology

Pu Xu

Shanghai Ocean University

Lixian Qiao

Qingdao Agricultural University

Bin Wang

Institute of Atmospheric Physics

Hongyu Liu

National Institute of Aerospace

Bin Wang

Central South University

Ge Zhao

China Medical University

Jixun Dai

University of Arizona

Cooperative Institutions

Chinese Academy of Sciences

Institute of Genetics and Developmental Biology

Ocean University of China

Institute of Oceanology

Peking University

Qingdao Agricultural University

University of Chinese Academy of Sciences

Qingdao National Laboratory for Marine Science and Technology

State Key Laboratory of Plant Genomics

Huazhong University of Science and Technology

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Molecular identification of 16 Porphyra lines using sequence-related amplified polymorphism markers

Aquatic Botany (2007)

Lixian Qiao Hongyu Liu Baotai Guo Manli Weng Jixun Dai

Porphyra

UPGMA

Primer (cosmetics)

Molecular marker

Dendrogram

Germ plasm

10.1016/j.aquabot.2007.06.006

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Citations (25)

Identification of 27 Porphyra lines (Rhodophyta) by DNA fingerprinting and molecular markers

Journal of Applied Phycology (2005)

Manli Weng Bo Liu Demin Jin Yang Qingkai Ge Zhao

Porphyra

DNA profiling

Identification

Germ plasm

10.1007/s10811-005-4845-8

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Citations (24)

Development of EST-SSR primers and their practicability test for Laminaria

Acta Oceanologica Sinica (2011)

Guo‐Liang Wang Xiaolong Tan Junling Shen Jian Li Lei Zhang

Saccharina

Primer (cosmetics)

10.1007/s13131-011-0125-4

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Citations (12)

Obtaining of SCAR markers for germplasm identification of Porphyra lines

Bin Sun Jin Demin Manli Weng Pu Xu Delin Duan

Randomly amplified polymorphic DNA (RAPD) technique is applied to studying the polymorphism of 27 Porphyra lines with free-living conchocelises as materials. All together, 13 Porphyra line-specific RAPD markers from 12 Porphyra lines were generated and they were cloned into pGEM-T easy vector respectively. Five of them from 5 important Porphyra lines were successfully sequenced. Five pairs of SCAR-PCR primers were then designed and synthesized according to the 5-terminal nucleotide sequences of the 5 RAPD markers. Through optimizing the reaction conditions, the specific RAPD markers of P. tenuipedalis and P. yezoensis Yqd-8 were successfully converted into SCAR markers. These will provide a useful way for Porphyra germplasm identification and protection.

Porphyra

Germ plasm

Source

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Application of Target Region Amplification Polymorphism (TRAP) Technique to Porphyra (Bangiales, Rhodophyta) Fingerprinting

Phycologia (2007)

Lixian Qiao Hongyu Liu Jianwei Sun Feng Zhao Baotai Guo

Abstract To demonstrate the applicability of the target region amplification polymorphism (TRAP) marker technique to Porphyra, 15 Porphyra lines, representing three species, were fingerprinted with 32 primer-combinations. Ten primer-combinations that gave a stable and reproducible amplification pattern were selected. In total 432 fragments from 50 to 500 base pairs (bp) in length were amplified with these 10 selected primer-combinations, and all the fragments were polymorphic. The 432 fragments were scored respectively and used to construct a dendrogram of the 15 Porphyra lines with unweighted pair-group method arithmetic average (UPGMA). These Porphyra lines were divided into two major groups at the 0.71 similarity level. This result is basically consistent with the conventional Porphyra taxonomy. Twelve of the 432 fragments, which were amplified by two primer-combinations, Tps/1F and Tps/3F, were chosen and used to develop the DNA fingerprints of these Porphyra lines. The developed DNA fingerprints then...

Porphyra

10.2216/07-08.1

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Citations (11)

The mutation induced by space conditions inEscherichia coli

World Journal of Gastroenterology (1999)

Manli Weng

Progress has been made in microorganism breeding under space conditions by boarding on recoverable satellite and high altitude balloon in China. To further study the mutagenesis in space, three strains of E. coli were put on board the recoverable satellite (JB1-B9611020) launched in October. 1996. After the satellite returned to the earth, the survival and mutation frequencies were determined and the results were discussed as well.

Mutation frequency

Mutation Breeding

10.3748/wjg.v5.i5.445

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Citations (5)

Development of SSR primers from EST sequences and their application in germplasm identification ofPorphyralines (Rhodophyta)

European Journal of Phycology (2006)

Jianwei Sun Tao Liu Baotai Guo Demin Jin Manli Weng

This paper reports the development of SSR markers from EST data and their utilization in germplasm identification of Porphyra. The publicly available EST (expressed sequence tag) sequences of Porphyra were searched from the Internet (http://www.kazura.or.jp/en/plant/porphyra/EST/). From a total of 20,779 obtained EST sequences, 391 SSRs (simple sequence repeats) were analysed with SSRIT software (http://www.gramene.org/db/searches/ssrtool). From those, 48 SSR primer-pairs were designed and tested by commonly used SSR reaction conditions using 22 Porphyra DNA samples as templates. Results showed that 41 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic diversity and variety identification of the 22 Porphyra lines. A dendrogram and the DNA fingerprints of the Porphyra lines were developed based on the obtained SSR data.

Porphyra

Germ plasm

Primer (cosmetics)

Tenera

Dendrogram

Identification

10.1080/09670260600740906

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Citations (25)

Expressed sequence tag analysis and cloning of trehalose-6-phosphate synthase gene from marine alga Laminaria japonica (Phaeophyta)

Acta Oceanologica Sinica (2012)

Jinsong Xuan Yanbin Feng Manli Weng Ge Zhao Jinfeng Shi