In this paper, we report U-Pb age and Hf isotope composition of hydrothermal zircons from a quartz vein within an UHP eclogite outcrop from the Hong’an area, western Dabie orogen. These data are used to decipher the age, formation conditions and source of fluid flow during the exhumation of UHP rock. Zircons from the vein have perfect euhedral shape, oscillatory zoning or weak zoning, and very low Th/U ratios (0.03―0.07), indicating that they precipitated from the aqueous fluid responsible for the vein formation. The weighted mean 206Pb/238U age of 224.7 ± 1.3 Ma of these zircons is taken as the best estimated age of the quartz vein formation, and records aqueous fluid flow during the early exhumation stage of the UHP rocks. The zircons in the quartz vein show low Lu/Hf ratios and have similar Hf compositions to those in the host eclogite, indicating an internal source and small-scale fluid transport for veining. Therefore, the Hf isotope composition of hydrothermal zircon from quartz vein can constrain on the nature, forming condition, and origin of high-pressure metamorphic fluids.