Lu Yu

University of Science and Technology of China

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Changlin Tian

University of Science and Technology of China

Quan Wang

Harvard University

Luke W. Guddat

University of Queensland

Yanting Tang

Nankai University

Sin Man Lam

Institute of Genetics and Developmental Biology

Hongri Gong

Nankai University

Guanghou Shui

Institute of Genetics and Developmental Biology

Zihe Rao

Tsinghua University

Changlin Tian

Hefei Institutes of Physical Science

Yan Gao

ShanghaiTech University

Cooperative Institutions

Chinese Academy of Sciences

University of Science and Technology of China

University of Chinese Academy of Sciences

Nanjing University

Nankai University

Tsinghua University

ShanghaiTech University

High Magnetic Field Laboratory

Shanghai Jiao Tong University

Hefei National Center for Physical Sciences at Nanoscale

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Combined approaches of EPR and NMR illustrate only one transmembrane helix in the human IFITM3

Scientific Reports (2016)

Shenglong Ling Chengwei Zhang Wei Wang Xiaoying Cai Lu Yu

Abstract Interferon-inducible transmembrane protein IFITM3 was known to restrict the entry of a wide spectrum of viruses to the cytosol of the host. The mechanism used by the protein to restrict viral entry is unclear given the unavailability of the membrane topology and structures of the IFITM family proteins. Systematic site-directed spin labeling (SDSL) and electron paramagnetic resonance (EPR) studies of IFITM3 in detergent micelles identified a single, long transmembrane helix in the C-terminus and an intramembrane segment in the N-terminal hydrophobic region. Solution NMR studies of the same sample verified the secondary structure distribution and demonstrated two rigid regions interacting with the micellar surface. The resulting membrane topology of IFITM3 supports the mechanism of an enhanced restricted membrane hemi-fusion.

Site-directed spin labeling

Helix (gastropod)

Membrane topology

Unavailability

10.1038/srep24029

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Citations (55)

UV-Vis Spectral Characteristic and Colour Characterization of Turquoise from Tongling, Anhui Province

Journal of Gems & Gemmology (2020)

Rui Zuo Hui Dai Wenqing Huang Lu Yu Wanghui Deng

In this paper, UV-Vis spectrometer, infrared spectrometer and other methods of gemmological analysis were used to study the micropscopic characteristics, UV-Vis spectra, infrared spectra and colour characterization of the turquoise samples from Tongling, Anhui Province. The results showed that the UV-Vis spectra of the turquoise samples from Tongling reveals 3+ absorption at 421, 429 nm, and the wide absorption band of the yellow-green area near the near infrared region is caused by 2+. As the main wavelength of the turquoise samples becomes smaller, the wide absorption band caused by 2+ moves towards the short wave direction. In addition, the brightness value of the turquoise samples from Tongling is negatively correlated with the chroma value when the main wavelength is similar. The results showed that the turquoise samples from Tongling have the same infrared spectra characteristic as turquoises from the other origins, and there is only a small deviation in a few wavenumbers which indicates that the molecular composition of the turquoise samples from Tongling is the same as turquoises from the other origins.

Characterization

10.15964/j.cnki.027jgg.2020.01.002

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Architecture of the mycobacterial succinate dehydrogenase with a membrane-embedded Rieske FeS cluster

Proceedings of the National Academy of Sciences (2021)

Xiaoting Zhou Yan Gao Weiwei Wang Xiaolin Yang Xiuna Yang

Significance Targeting energy metabolism in Mycobacterium tuberculosis has emerged as a new paradigm in antituberculosis drug discovery. Succinate dehydrogenase is considered the regulator of respiration in M. tuberculosis . Mycobacteria contains two different succinate dehydrogenase enzymes designated Sdh1 and Sdh2. Sdh1 has recently been identified as a new class of succinate dehydrogenase. In this study, we have determined M. smegmatis Sdh1 structures alone and in the presence of ubiquinone-1, revealing that Sdh1 has a novel electron transfer pathway and a unique substrate-binding site. These data show that the structure of M. tuberculosis Sdh1 is significantly different by comparison with the human counterpart making a good antituberculosis drug target.

Mycobacterium smegmatis

10.1073/pnas.2022308118

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Author Correction: Cryo-EM structure of trimeric Mycobacterium smegmatis succinate dehydrogenase with a membrane-anchor SdhF

Nature Communications (2021)

Hongri Gong Yan Gao Xiaoting Zhou Yu Xiao Weiwei Wang

A Correction to this paper has been published: https://doi.org/10.1038/s41467-021-21616-3

Mycobacterium smegmatis

10.1038/s41467-021-21616-3

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An electron transfer path connects subunits of a mycobacterial respiratory supercomplex

Science (2018)

Hongri Gong Jun Li Ao Xu Yanting Tang Wenxin Ji

An electron bridge in place of a ferry Respiratory complexes are massive, membrane-embedded scaffolds that position redox cofactors so as to permit electron transfer coupled to the movement of protons across a membrane. Gong et al. used cryo–electron microscopy to determine a structure of a stable assembly of mycobacterial complex III–IV, in which a complex III dimer is sandwiched between two complex IV monomers. A potential direct electron transfer path stretches from the quinone oxidizing centers in complex III to the oxygen reduction centers in complex IV. A loosely associated superoxide dismutase may play a role in detoxifying superoxide produced from uncoupled oxygen reduction. Science , this issue p. eaat8923

Mycobacterium smegmatis

10.1126/science.aat8923

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Citations (150)

Probing the dynamic properties in the LLPS process via site-directed spin labeling-electron paramagnetic resonance (SDSL-EPR) spectroscopy

Chinese Chemical Letters (2024)

Ruotong Wei Aokun Liu Jian Kuang Zhiwen Wang Lu Yu

Site-directed spin labeling

Pulsed EPR

10.1016/j.cclet.2024.110029

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2.8 μm emission and OH quenching analysis in Ho3+ doped fluorotellurite-germanate glasses sensitized by Yb3+ and Er3+

Scientific Reports (2017)

Junjie Zhang Lu Yu Muzhi Cai Ying Tian Feifei Huang

The use of Yb3+ and Er3+ co-doping with Ho3+ to enhance and broaden the Ho3+: 5I6 → 5I7 ~2.8 μm emissions are investigated in the fluorotellurite-germanate glasses. An intense ~3 μm emission with a full width at half maximum (FWHM) of 245 nm is achieved in the Er3+/Ho3+/Yb3+ triply-doped fluorotellurite-germanate glass upon excitation at 980 nm. The glass not only possesses considerably low OH- absorption coefficient (0.189 cm-1), but also exhibits low phonon energy (704 cm-1). Moreover, the measured lifetime of Ho3+: 5I6 level is as high as 0.218 ms. In addition, the energy transfer rate to hydroxyl groups and quantum efficiency (η) of 5I6 level were calculated in detail by fitting the variations of lifetimes vs. the OH- concentrations. The formation ability and thermal stability of glasses have been improved by introducing GeO2 into fluorotellurite glasses. Results reveal that Er3+/Ho3+/Yb3+ triply-doped fluorotellurite-germanate glass is a potential kind of laser glass for efficient 3 μm laser.

Germanate

10.1038/s41598-017-16937-7

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Electron spin resonance analysis of photoenzymatic catalysis

National Science Review (2024)

Lu Yu Changlin Tian

This Perspective highlights recent research progress and prospects in elucidating the catalytic mechanism of photoenzymes using ESR (electron spin resonance) spectroscopy, which is emerging as a unique and crucial method for identifying radical intermediates, illustrating electron transfer events and the underlying mechanisms of photoenzymatic catalysis.

Site-directed spin labeling

10.1093/nsr/nwae095

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Helicobacter pylori FabX contains a [4Fe-4S] cluster essential for unsaturated fatty acid synthesis

Nature Communications (2021)

Jiashen Zhou Lin Zhang Liping Zeng Lu Yu Yuanyuan Duan

Abstract Unsaturated fatty acids (UFAs) are essential for functional membrane phospholipids in most bacteria. The bifunctional dehydrogenase/isomerase FabX is an essential UFA biosynthesis enzyme in the widespread human pathogen Helicobacter pylori , a bacterium etiologically related to 95% of gastric cancers. Here, we present the crystal structures of FabX alone and in complexes with an octanoyl-acyl carrier protein (ACP) substrate or with holo-ACP. FabX belongs to the nitronate monooxygenase (NMO) flavoprotein family but contains an atypical [4Fe-4S] cluster absent in all other family members characterized to date. FabX binds ACP via its positively charged α7 helix that interacts with the negatively charged α2 and α3 helices of ACP. We demonstrate that the [4Fe-4S] cluster potentiates FMN oxidation during dehydrogenase catalysis, generating superoxide from an oxygen molecule that is locked in an oxyanion hole between the FMN and the active site residue His182. Both the [4Fe-4S] and FMN cofactors are essential for UFA synthesis, and the superoxide is subsequently excreted by H. pylori as a major resource of peroxide which may contribute to its pathogenic function in the corrosion of gastric mucosa.

Flavoprotein

10.1038/s41467-021-27148-0

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Citations (14)

CW-EPR studies revealed different motional properties and oligomeric states of the integrin β1a transmembrane domain in detergent micelles or liposomes

Scientific Reports (2015)

Lu Yu Wei Wang Shenglong Ling Sanling Liu Liang Xiao

Integrins are heterodimeric membrane proteins that regulate essential processes: cell migration, cell growth, extracellular matrix assembly and tumor metastasis. Each integrin α or β subunit contains a large extracellular domain, a single transmembrane (TM) domain and a short cytoplasmic tail. The integrin TM domains are important for heterodimeric association and dissociation during the conversion from inactive to active states. Moreover, integrin clustering occurs by homo-oligomeric interactions between the TM helices. Here, the transmembrane and cytoplasmic (TMC) domains of integrin β1a were overexpressed and the protein was purified in detergent micelles and/or reconstituted in liposomes. To investigate the TM domain conformational properties of integrin β1a, 26 consecutive single cysteine mutants were generated for site-directed spin labeling and continuous-wave electron paramagnetic resonance (CW-EPR) mobility and accessibility analyses. The mobility analysis identified two integrin β1a-TM regions with different motional properties in micelles and a non-continuous integrin β1a-TM helix with high immobility in liposomes. The accessibility analysis verified the TM range (Val737-Lys752) of the integrin β1a-TMC in micelles. Further mobility and accessibility comparisons of the integrin β1a-TMC domains in micelles or liposomes identified distinctively different oligomeric states of integrin β1a-TM, namely a monomer embedded in detergent micelles and leucine-zipper-like homo-oligomeric clusters in liposomes.

10.1038/srep07848

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Citations (29)