Travertine deposits in Deir Alla, Suwayma, and Az Zara areas were investigated. Mineralogy, geochemistry, stable isotopes and age dating indicate the presence of low-Mg calcite, with minor quartz components. The variable isotope (δ13C and δ18O) signatures indicate dependence on water temperature and water/rock isotopic exchange. In contrast, the high δ13C values in some travertine samples reflect 12CO2 degassing processes, increased input of 13C-enriched groundwater, and the presence of surface and groundwater hydrological systems. The high δ18O values may be attributed to evaporation effects and low water temperature during the formation of localized travertine. The age of travertine is the Late Pleistocene.