Rhizoremediation is a promising remediation technology for the removal of soil persistent organic pollutants (POPs), especially benzo[a]pyrene (BaP). However, our understanding of the associations among rhizospheric soil metabolites, functional microorganisms, and POPs degradation in different plant growth stages is limited. We combined stable-isotope probing (SIP), high-throughput sequencing, and metabolomics to analyze changes in rhizospheric soil metabolites, functional microbes, and BaP biodegradation in the early growth stages (tillering, jointing) and later stage (booting) of ryegrass. Microbial community structures differed significantly among growth stages. Metabolisms such as benzenoids and carboxylic acids tended to be enriched in the early growth stage, while lipids and organic heterocyclic compounds dominated in the later stage. From SIP, eight BaP-degrading microbes were identified, and most of which such as Ilumatobacter and Singulisphaera were first linked with BaP biodegradation. Notably, the relationship between the differential metabolites and BaP degradation efficiency further suggested that BaP-degrading microbes might metabolize BaP directly to produce benzenoid metabolites (3-hydroxybenzo[a]pyrene), or utilize benzenoids (phyllodulcin) to stimulate the co-metabolism of BaP in early growth stage; some lipids and organic acids, e.g. 1-aminocyclopropane-1-carboxylic acid, might provide nutrients for the degraders to promote BaP metabolism in later stage. Accordingly, we determined that certain rhizospheric metabolites might regulate the rhizospheric microbial communities at different growth stages, and shift the composition and diversity of BaP-degrading bacteria, thereby enhancing in situ BaP degradation. Our study sheds light on POPs rhizoremediation mechanisms in petroleum-contaminated soils.
This study reports the transformation of nitrogen during liquefaction of two microalgae (i.e., Chlorella vulgaris and Spirulina) in subcritical/supercritical ethanol at 220–260 °C. The nitrogen contents in all products from microalgae liquefaction were quantified, and the nitrogen species in those products were characterized. The nitrogen in the raw microalgae are mainly present in the form of protein-N, with a small fraction of nitrogen present in other forms such as quaternary-N, inorganic-N, and pyridinic-N/amine-N. The nitrogen in the solid residue is found to decrease significantly at 220 °C. As the liquefaction temperature further increases, the nitrogen in char gradually decreases, accompanied by the increase of nitrogen in bio-oil, i.e., up to 83.2% at 260 °C for Chlorella vulgaris and 90.1% at 250 °C for Spirulina. The results demonstrate that the nitrogen species in the raw microalgae are not stable and easily transformed into other N-containing species in bio-oil (mainly the pyrrole and amine species). A small fraction of pyrrolic-N is also formed in the solid residue at 220 °C, while the formation of N-containing gas species is negligible.
The photoactive metal-organic frameworks (MOFs) are good candidates for photocatalysts, but the quick electron-hole pairs recombination has greatly restricted the photocatalytic ability of MOFs. To improve the photoactivity of MOFs, MOFs-based composite materials have been extensively studied. Here, we successfully integrated MoS2 quantum dots (QDs) with UiO-66-NH2 for the first time under hydrothermal conditions. The as-prepared MoS2 QDs/UiO-66-NH2 (MS-U) had good visible light response ability (absorption edge at 445 nm), and charge separation and transfer ability, which lays the foundation for the photocatalytic Cr(VI) reduction. Photocatalytic studies revealed that MoS2 QDs-5/UiO-66-NH2 (MS-U-5) had superior Cr(VI) reduction activity than pure MoS2 QDs and UiO-66-NH2. MS-U-5 could remove 98% Cr(VI) at pH= 2 with visible light irradiation for 20 min, which is the fastest visible light driven Cr(VI) reduction rate among the reported MOFs-based composite photocatalysts without the presence of any cocatalysts or scavengers as far as we know. Importantly, MS-U-5 could be reused at least three times. In the end, the possible electron transfer path and mechanism of Cr(VI) reduction was also investigated.
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