Polar environments are exposed to the risk of oil pollution. However, there is limited knowledge regarding how the variation of physicochemical factors influencing biodegradation may affect bacterial community structure. The effects of temperature (4, 10 and 20°C) and organic fertilization (Inipol EAP 22) on community structure and diversity of bacteria inhabiting Kerguelen sub-Antarctic waters were studied in crude- and diesel-amended microcosms. Dynamics of total (i.e., 16S rDNA-based) and metabolically active (i.e., 16S rRNA-based) bacterial community structure and diversity were monitored using capillary-electrophoresis single-strand conformation polymorphism. Results showed that total and active community structures were differently influenced by temperature and fertilization in the presence of hydrocarbons. Both fertilization and temperature induced changes in total community structure in the presence of crude oil and diesel. However, temperature showed a limited influence on active community structure, and fertilization induced changes in the presence of crude oil only. Simpson's index decreased for total bacterial communities at all temperatures in the presence of crude oil and diesel, whereas a lower reduction was observed for active bacterial populations. In the presence of fertilizer, the diversity of the whole community approached control values after seven incubation weeks; this was not observed for the active bacterial community. This study evidenced qualitative differences in total and active bacterial community structures of Kerguelen seawaters in the presence of hydrocarbons and different responses relative to variation in temperature and fertilization. These factors and hydrocarbons composition have to be taken into account to understand bacterial community dynamics after an oil spill.Keywords: Oil hydrocarbons; Inipol EAP 22; temperature; 16S rDNA/rRNA; sub-Antarctic seawaterCitation: Polar Research 2013, 32, 18521, http://dx.doi.org/10.3402/polar.v32i0.18521
Oxygen depletion in the 100‐m thick bottom layer of the deep Lower St. Lawrence Estuary is currently thought to be principally caused by benthic oxygen demand overcoming turbulent oxygenation from overlying layers, with pelagic respiration playing a secondary role. This conception is revisited with idealized numerical simulations, historical oxygen observations and new turbulence measurements. Results indicate that a dominant sediment oxygen demand, over pelagic, is incompatible with the shape of observed oxygen profiles. It is further argued that to sustain oxygen depletion, the turbulent diffusivity in the bottom waters should be ≪10 −4 m 2 s −1 , consistent with direct measurements but contrary to previous model results. A new model that includes an Arrhenius‐type function for pelagic respiration and a parameterization for turbulence diffusivity is developed. The model demonstrates the importance of the bottom boundary layer in reproducing the shape of oxygen profiles and reproduces to within 14% the observed change in oxygen concentration in the Lower St. Lawrence Estuary. The analysis indicates that turbulent oxygenation represents about 8% of the sum of sediment and pelagic oxygen demand, consistent with the low turbulent oxygenation required to maintain oxygen depletion. However, contrary to previous hypotheses, it is concluded that pelagic oxygen demand needs to be five time larger than sediment oxygen demand to explain hypoxia in the 100‐m thick bottom layer of the Lower St. Lawrence Estuary.
Due to the scanty description of Porambonites dentata Pander, 1830 and loss of its single type specimen, the name dentata has been subsequently attributed to various Ordovician to Silurian species of the genus Platystrophia s.l. with two costae in the sulcus and three on the fold.In The Natural History Museum, London, there is a complete shell identified presumably by Christian Pander himself as Spirifer dentatus from Pulkowa.That specimen is selected here as neotype to Platystrophia dentata, i.e. to a species, which on the basis of new material is restricted to the lower Darriwilian in the St Petersburg region.Considering differences in the interior of the dorsal valve, the other species (some with subspecies) of the so-called dentata-group, especially from the stratigraphically younger strata, are discussed and excluded from the material described here of P. dentata.