Congli Cai

Chinese Academy of Medical Sciences & Peking Union Medical College

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Renxian Wang

Peking University

Longchang Xu

Chinese Academy of Medical Sciences & Peking Union Medical College

Wei Song

National Institutes of Health

Junbo Liang

Chinese Academy of Medical Sciences & Peking Union Medical College

Shan Lu

Chinese Academy of Medical Sciences & Peking Union Medical College

Linfang Wang

Shanxi Agricultural University

Shiying Miao

Chinese Academy of Medical Sciences & Peking Union Medical College

Cooperative Institutions

Chinese Academy of Medical Sciences & Peking Union Medical College

Peking Union Medical College Hospital

Jingdong (China)

Peking University

Beijing Jishuitan Hospital

Capital Medical University

Weatherford College

University of Hong Kong

Beijing Administration Institute

Northwestern Polytechnical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Small Kinetochore Associated Protein (SKAP) Promotes UV-Induced Cell Apoptosis through Negatively Regulating Pre-mRNA Processing Factor 19 (Prp19)

PLoS ONE (2014)

Shan Lu Renxian Wang Congli Cai Junbo Liang Longchang Xu

Apoptosis is a regulated cellular suicide program that is critical for the development and maintenance of healthy tissues. Previous studies have shown that small kinetochore associated protein (SKAP) cooperates with kinetochore and mitotic spindle proteins to regulate mitosis. However, the role of SKAP in apoptosis has not been investigated. We have identified a new interaction involving SKAP, and we propose a mechanism through which SKAP regulates cell apoptosis. Our experiments demonstrate that both overexpression and knockdown of SKAP sensitize cells to UV-induced apoptosis. Further study has revealed that SKAP interacts with Pre-mRNA processing Factor 19 (Prp19). We find that UV-induced apoptosis can be inhibited by ectopic expression of Prp19, whereas silencing Prp19 has the opposite effect. Additionally, SKAP negatively regulates the protein levels of Prp19, whereas Prp19 does not alter SKAP expression. Finally, rescue experiments demonstrate that the pro-apoptotic role of SKAP is executed through Prp19. Taken together, these findings suggest that SKAP promotes UV-induced cell apoptosis by negatively regulating the anti-apoptotic protein Prp19.

Ectopic expression

10.1371/journal.pone.0092712

Cite

Citations (8)