José Augusto Pires Bitencourt

Vale Technological Institute

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Mírian Araújo Carlos Crapez

Universidade Federal Fluminense

Guilherme Oliveira

Vale Technological Institute

Markus Gastauer

Vale Technological Institute

Carolina Coelho da Costa Waite

Universidade Federal Fluminense

Lígia Carla Faccin‐Galhardi

Universidade Estadual de Londrina

Leandro Viana Guerra

Universidade Federal Fluminense

Sebastião da Cruz Silva

Universidade Federal do Sul e Sudeste do Pará

ÉR

Érica Romão Pereira

Universidade Estadual de Londrina

Rayara do Socorro Souza da Silva

Universidade Federal do Pará

Elisamara Sabadini-Santos

Universidade Federal Fluminense

Cooperative Institutions

Vale Technological Institute

Universidade Federal Fluminense

Universidade Estadual de Londrina

Vale (Brazil)

Universidade Federal do Rio de Janeiro

Instituto de Desenvolvimento Sustentável Mamirauá

Universidade de São Paulo

Universidade Federal do Pará

University of Queensland

Universidade Federal do Sul e Sudeste do Pará

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Copper and lead removal from aqueous solutions by bacterial consortia acting as biosorbents

Marine Pollution Bulletin (2016)

Carolina Coelho da Costa Waite Guilherme Oliveira Andrade da Silva José Augusto Pires Bitencourt Elisamara Sabadini-Santos Mírian Araújo Carlos Crapez

Biosorption

Efflux

Esterase

10.1016/j.marpolbul.2016.05.044

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Citations (28)

Potential application of Pseudomonas stutzeri W228 for removal of copper and lead from marine environments

PLoS ONE (2020)

Carolina Coelho da Costa Waite Guilherme Oliveira Andrade da Silva José Augusto Pires Bitencourt Luciana Pereira Torres Chequer Simone Pennafirme

High concentrations of metals in the environment alter bacterial diversity, selecting resistant and tolerant species. The study evaluated the selection of a potential bacterial strain from Sepetiba Bay-Rio de Janeiro, Brazil marine sediments to remove Cu and Pb. The bacterial strain isolated from the sediments was used in three different bioassays: (1) Cu at concentrations of 0 (control), 6 and 50 μg.mL-1; (2) Pb at concentrations of 0 (control), 6 and 50 μg.mL-1; (3) Cu + Pb in concentrations of 3 μg.mL-1 Cu + 3 μg.mL-1 Pb (6 μg.mL-1) and 25 μg.mL-1 Cu + 25 μg.mL-1 Pb (50 μg.mL-1). The number of cells and the enzymatic activities of dehydrogenases and esterases were quantified. Results of taxonomic identification indicated the selection of the Pseudomonas stutzeri W228 strain, showing a greater degree of similarity (±73%) with the database used. There was no significant variation in the number of cells, 108 cells.mL-1, which represents a high biomass production in the presence of stressors. However, we observed a reduction in dehydrogenase activity at all tested concentrations of Cu, Pb and Cu + Pb. The activity of esterase increased, indicating a higher energy demand to complete the bacterial life cycle. The study showed significant results for the absorption of Pb by the extracellular polymeric substances (EPS) and the efflux of Cu. The capacity of Pb absorption by EPS can be considered a resistance mechanism, as well as the efflux of Cu, so that the available EPS sites could be occupied by the most toxic ions demonstrating that Pseudomonas stutzeri is resistant to Pb and Cu.

Pseudomonas stutzeri

Efflux

Strain (injury)

10.1371/journal.pone.0240486

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Citations (13)

Acquiring Iron-Reducing Enrichment Cultures: Environments, Methods and Quality Assessments

Microorganisms (2023)

Aline Figueiredo Cardoso Rayara do Socorro Souza da Silva Isabelle Gonçalves de Oliveira Prado José Augusto Pires Bitencourt Markus Gastauer

Lateritic duricrusts cover iron ore deposits and form spatially restricted, unique canga ecosystems endangered by mining. Iron cycling, i.e., the dissolution and subsequent precipitation of iron, is able to restitute canga duricrusts, generating new habitats for endangered biota in post-mining landscapes. As iron-reducing bacteria can accelerate this iron cycling, we aim to retrieve microbial enrichment cultures suitable to mediate the large-scale restoration of cangas. For that, we collected water and sediment samples from the Carajás National Forest and cultivated the iron-reducing microorganisms therein using a specific medium. We measured the potential to reduce iron using ferrozine assays, growth rate and metabolic activity. Six out of seven enrichment cultures effectively reduced iron, showing that different environments harbor iron-reducing bacteria. The most promising enrichment cultures were obtained from environments with repeated flooding and drying cycles, i.e., periodically inundated grasslands and a plateau of an iron mining waste pile characterized by frequent soaking. Selected enrichment cultures contained iron-reducing and fermenting bacteria, such as Serratia and Enterobacter. We found higher iron-reducing potential in enrichment cultures with a higher cell density and microorganism diversity. The obtained enrichment cultures should be tested for canga restoration to generate benefits for biodiversity and contribute to more sustainable iron mining in the region.

Biota

Enrichment culture

Iron bacteria

Direct reduced iron

10.3390/microorganisms11020448

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Citations (5)

Biogeochemical processes in canga ecosystems: Armoring of iron ore against erosion and importance in iron duricrust restoration in Brazil

Ore Geology Reviews (2019)

Emma J. Gagen Alan Levett Anat Paz Markus Gastauer Cecílio Fróis Caldeira

Biogeochemical Cycle

Iron ore

Cementation (geology)

10.1016/j.oregeorev.2019.03.013

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Citations (38)

Evaluation of the sensitivity to zinc of ciliates Euplotes vannus and Euplotes crassus and their naturally associated bacteria isolated from a polluted tropical bay

Environmental Science and Pollution Research (2014)

José Augusto Pires Bitencourt Daniella Cristina Rodrigues Pereira Inácio Domingos da Silva‐Neto Mírian Araújo Carlos Crapez

10.1007/s11356-014-3828-1

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Citations (4)

Bioavailability of Organic Matter in the Superficial Sediment of Guanabara Bay, Rio de Janeiro, Brazil

DOAJ (DOAJ: Directory of Open Access Journals) (2011)

José Augusto Pires Bitencourt Frederico Sobrinho da Silva Leandro Viana Guerra Mírian Araújo Carlos Crapez José Antônio Baptista-Neto

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Comparison of two acidophilic sulfidogenic consortia for the treatment of acidic mine water

Frontiers in Bioengineering and Biotechnology (2022)

Tayná Diniz Frederico Iván Ñancucheo Werica Colaço Barros Santos Renato Oliveira Daniella Cardoso Buzzi

Sulfate-reducing bioreactors are a biotechnological alternative for the treatment of acid mine drainage (AMD). In this study, two separate bioreactors with pH and temperature-controlled (Bio I and II) were operated with two different acidophilic microbial consortia to determine their efficiencies in sulfate removal from a synthetic acidic mine water. The bioreactors were operated for 302 days in continuous flow mode under the same parameters: fed with a sulfate solution of ∼30 mM with a pH of 2.5, the temperature at 30°C, stirred gently at 40 rpm and using a continuous stream of nitrogen to help remove the H2S produced in the bioreactor. The glycerol consumption, acetate production, and sulfate removal were monitored throughout the course of the experiment. The community composition and potential metabolic functional groups were analyzed via 16S rRNA partial gene sequencing. Bio I consortium reduced the sulfate, achieving a range of sulfate concentration from 4.7 to 19 mM in the effluent liquor. The removal of sulfate in Bio II was between 5.6 and 18 mM. Both bioreactors' communities showed the presence of the genus Desulfosporosinus as the main sulfate-reducing bacteria (SRB). Despite differences in microbial composition, both bioreactors have similar potential metabolism, with a higher percentage of microorganisms that can use sulfate in respiration. Overall, both bioreactors showed similar performance in treating acidic mine water containing mostly sulfate using two different acidophilic sulfidogenic consortia obtained from different global locations.

Acid Mine Drainage

Sulfate-Reducing Bacteria

Microbial consortium

10.3389/fbioe.2022.1048412

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Citations (3)

Production and characterization of rhamnolipids by Pseudomonas aeruginosa isolated in the Amazon region, and potential antiviral, antitumor, and antimicrobial activity

Scientific Reports (2024)

Sidnei Cerqueira dos Santos Chayenna Araújo Torquato Darlisson de Alexandria Santos Alexandre Orsato Karoline Leite

Abstract Biosurfactants encompass structurally and chemically diverse molecules with surface active properties, and a broad industrial deployment, including pharmaceuticals. The interest is growing mainly for the low toxicity, biodegradability, and production from renewable sources. In this work, the optimized biosurfactant production by Pseudomonas aeruginosa BM02, isolated from the soil of a mining area in the Brazilian Amazon region was assessed, in addition to its antiviral, antitumor, and antimicrobial activities. The optimal conditions for biosurfactant production were determined using a factorial design, which showed the best yield (2.28 mg/mL) at 25 °C, pH 5, and 1% glycerol. The biosurfactant obtained was characterized as a mixture of rhamnolipids with virucidal properties against Herpes Simplex Virus, Coronavirus, and Respiratory Syncytial Virus, in addition to antimicrobial properties against Gram-positive bacteria ( Staphylococcus aureus and Enterococcus faecium ), at 50 µg/mL. The antitumor activity of BS (12.5 µg/mL) was also demonstrated, with potential selectivity in reducing the proliferation of breast tumor cells, after 1 min of exposure. These results demonstrate the importance of studying the interconnection between cultivation conditions and properties of industrially important compounds, such as rhamnolipid-type biosurfactant from P. aeruginosa BM02, a promising and sustainable alternative in the development of new antiviral, antitumor, and antimicrobial prototypes.

Rhamnolipid

Enterococcus faecium

10.1038/s41598-024-54828-w

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Citations (6)