Abstract The Caledonides of Ny Friesland comprise the type Hecla Hoek sequence of Svalbard, a succession of late Proterozoic to Ordovician strata greater than 18 km thick. Three supergroups constitute the sequence: the Stubendorffbreen Supergroup (Riphean), the Lomfjorden Supergroup (late Riphean-Sturtian) and the Hinlopenstretet Supergroup (Varanger-mid-Ordovician). Basement elements have recently been identified within the Stubendorffbreen Supergroup, but their extent and significance is yet to be established. The Stubendorffbreen Supergroup records the deposition of sediments and volcanics (both acid and basic) in an unstable marine environment. In contrast, the Lomfjorden and Hinlopenstretet supergroups record sedimentation in a shallow-marine, periodically emergent, stable environment without volcanism. The Ny Friesland Orogen is divided into two subterranes by the Veteranen Line, a zone of attenuation along which sinistral strike-slip displacement has occurred. This line separates the strongly deformed Stubendorffbreen Supergroup rocks in the west from the less-intensely deformed Lomfjorden and Hinlopenstretet supergroup rocks in the east. Despite these contrasts and the obvious displacement, there is no evidence that a significant stratigraphie break occurs across it. All the supergroups were deformed and metamorphosed during the late Ordovician-Silurian Ny Friesland Orogeny. Early compressional deformation produced isoclinal folding and nappes in the Stubendorffbreen Supergroup rocks, accompanied by amphibolite faciès metamorphism; deformation in the Lomfjorden and Hinlopenstretet supergroups was less intense with open, upright folds and greenschist or subgreenschist facies metamorphism. Early compression was followed by a Silurian transpressive deformation that generated a pervasive lineation in the Stubendorffbreen Supergroup rocks. Transpressive deformation and the associated sinistral strike-slip was focused where strata were in a near-vertical attitude conducive to displacement. At a late stage in the orogeny, and probably still under a strike-slip regime, batholiths were emplaced into rocks east of the Veteranen Line. As a result of continued sinistral displacement (transpression, transcurrence and transtension) along the Billefjorden Fault Zone, Ny Friesland (part of the Eastern Province of Svalbard) finally docked against the Central Province during the late Devonian Svalbardian movements. At the same time, the Central Province docked against the Western Province. In total, hundreds of kilometres of Caledonian displacement along the Billefjorden Fault Zone brought the Eastern and Central provinces into their present positions. Pre-Carboniferous Svalbard is thus a composite terrane of at least three provinces, each comprising more than one minor terrane.
The International Conferences on Contaminants in Freezing Ground are organised under the auspices of an International Steering Committee to promote a better understanding of the unique characteristics and problems posed by contaminants in freezing ground. The first meeting was held in Cambridge in 1997 and was attended by 33 participants from nine countries. Results from the meeting were reported in Polar Record in 1998 (volume 35). The themes covered at the first meeting reflected a broad range of interests, including a synthesis of the Arctic environmental strategy as it stood in 1997; the fundamental physical, chemical, and biological properties of contaminated frozen soils; experimental approaches to determining contaminant movement; and possibilities for in situ bioremediation of petroleum spills.
The soil substrate membrane system (SSMS) is a novel micro-culturing technique targeted at terrestrial soil systems. We applied the SSMS to pristine and diesel fuel spiked polar soils, along with traditional solid media culturing and culture independent 454 tag pyrosequencing to elucidate the effects of diesel fuel on the soil community. The SSMS enriched for up to 76% of the total soil diversity within high diesel fuel concentration soils, in contrast to only 26% of the total diversity for the control soils. The majority of organisms originally recovered with the SSMS were lost in the transfer to solid media, with all 300 isolates belonging to Proteobacteria, Firmicutes, Actinobacteria or Bacteroidetes, the four phyla most frequently associated with soil culturing efforts. The soils spiked with high diesel fuel concentrations exhibited reduced species richness, diversity and a selection towards heterotrophs and hydrocarbon degraders in comparison to the control soils. Based on these observations and the unusually high level of overlap in microbial taxa observed between methods, we suggest the SSMS holds potential to exploit hydrocarbon degraders and other targets within simplified bacterial systems, yet is inadequate for soil ecology and ecotoxicology studies where identifying rare oligotrophic species is paramount.
Despite decreasing costs, generating large-scale, well-replicated and multivariate microbial ecology investigations with sequencing remains an expensive and time-consuming option. As a result, many microbial ecology investigations continue to suffer from a lack of appropriate replication. We evaluated two fingerprinting approaches - terminal restriction fragment length polymorphism (T-RFLP) and automated ribosomal intergenic spacer analysis (ARISA) against 454 pyrosequencing, by applying them to 225 polar soil samples from East Antarctica and the high Arctic. By incorporating local and global spatial scales into the dataset, our aim was to determine whether various approaches differed in their ability and hence utility, to identify ecological patterns. Through the reduction in the 454 sequencing data to the most dominant OTUs, we revealed that a surprisingly small proportion of abundant OTUs (< 0.25%) was driving the biological patterns observed. Overall, ARISA and T-RFLP had a similar capacity as sequencing to separate samples according to distance at a local scale, and to correlate environmental variables with microbial community structure. Pyrosequencing had a greater resolution at the global scale but all methods were capable of significantly differentiating the polar sites. We conclude fingerprinting remains a legitimate approach to generating large datasets as well as a cost-effective rapid method to identify samples for elucidating taxonomic information or diversity estimates with sequencing methods.
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