Shengle Huang

Ghent University

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Ramon Ganigué

Ghent University

Korneel Rabaey

Ghent University

Pieter Candry

University of Washington

Robbert Kleerebezem

Delft University of Technology

Wei‐Shan Chen

Zhejiang University

Cees J.N. Buisman

Wageningen University & Research

José M. Carvajal‐Arroyo

Ghent University

David P. B. T. B. Strik

Wageningen University & Research

Caroline M. Plugge

Wageningen University & Research

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Wageningen University & Research

Ghent University

Centre for Advanced Process Technology for Urban Resource Recovery

Ghent University Hospital

Ecologie Microbienne Lyon

University of Queensland

University of Washington

Delft University of Technology

Cell Biotech (South Korea)

Biocat

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Open microbiome dominated by Clostridium and Eubacterium converts methanol into i-butyrate and n-butyrate

Applied Microbiology and Biotechnology (2020)

Shengle Huang Robbert Kleerebezem Korneel Rabaey Ramon Ganigué

Eubacterium

10.1007/s00253-020-10551-w

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Concurrent use of methanol and ethanol for chain-elongating short chain fatty acids into caproate and isobutyrate

Journal of Environmental Management (2020)

Wei‐Shan Chen Shengle Huang Caroline M. Plugge Cees J.N. Buisman David P. B. T. B. Strik

Microbial chain elongation (MCE) is a bioprocess that could utilise a mixed-culture fermentation to valorise organic waste. MCE converting ethanol and short chain fatty acids (SCFA; derived from organic waste) to caproate has been studied extensively and implemented. Recent studies demonstrated the conversion of SCFAs and methanol or ethanol into isomerised fatty acids as novel products, which may expand the MCE application and market. Integrating caproate and isomerised fatty acid production in one reactor system is theoretically feasible given the employment of a mixed culture and may increase the economic competence of MCE; however, the feasibility of such has never been demonstrated. This study investigated the feasibility of using two electron donors, i.e. methanol and ethanol, for upgrading SCFAs into isobutyrate and caproate concurrently in MCE Results show that supplying methanol and ethanol in MCE simultaneously converted acetate and/or butyrate into caproate and isobutyrate, by a mixed-culture microbiome. The butyrate supplement stimulated the caproate production rate from 1.5 to 2.6 g/L.day and induced isobutyrate production (1.5 g/L.day). Further increasing ethanol feeding rate from 140 to 280 mmol carbon per litre per day enhanced the direct use of butyrate for caproate production, which improved the caproate production rate to 5.9 g/L.day. Overall, the integration of two electron donors, i.e. ethanol and methanol, in one chain-elongation reactor system for upgrading SCFAs was demonstrated. As such, MCE could be applied to valorise organic waste (water) streams into a wider variety of value-added biochemical.

Sequencing batch reactor

Organic acid

10.1016/j.jenvman.2019.110008

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Enrichment and characterisation of ethanol chain elongating communities from natural and engineered environments

Scientific Reports (2020)

Pieter Candry Shengle Huang José M. Carvajal‐Arroyo Korneel Rabaey Ramon Ganigué

Abstract Chain elongation is a microbial process in which an electron donor, such as ethanol, is used to elongate short chain carboxylic acids, such as acetic acid, to medium chain carboxylic acids. This metabolism has been extensively investigated, but the spread and differentiation of chain elongators in the environment remains unexplored. Here, chain elongating communities were enriched from several inocula (3 anaerobic digesters, 2 animal faeces and 1 caproic acid producing environment) using ethanol and acetic acid as substrates at pH 7 and 5.5. This approach showed that (i) the inoculum’s origin determines the pH where native chain elongators can grow; (ii) pH affects caproic acid production, with average caproic acid concentrations of 6.4 ± 1.6 g·L −1 at pH 7, versus 2.3 ± 1.8 g·L −1 at pH 5.5; however (iii) pH does not affect growth rates significantly; (iv) all communities contained a close relative of the known chain elongator Clostridium kluyveri ; and (v) low pH selects for communities more enriched in this Clostridium kluyveri -relative (57.6 ± 23.2% at pH 7, 96.9 ± 1.2% at pH 5.5). These observations show that ethanol-consuming chain elongators can be found in several natural and engineered environments, but are not the same everywhere, emphasising the need for careful inoculum selection during process development.

Caproic Acid

Valeric acid

10.1038/s41598-020-60052-z

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Citations (35)