Mukesh Kumar Malviya

Guangxi Academy of Agricultural Science

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Manoj Kumar Solanki

University of Silesia in Katowice

Changning Li

Guangxi Academy of Agricultural Science

Yang‐Rui Li

Guangxi Academy of Agricultural Science

Jinlan Xie

Guangxi Academy of Agricultural Science

Qian Nong

Guangxi Academy of Agricultural Science

Pratiksha Singh

National Dairy Research Institute

Rajesh Kumar Singh

Central Agricultural University

Krishan K. Verma

Guangxi Academy of Agricultural Science

Reemon Htun

Mandalay Technological University

Xiaoyan Liu

Zhejiang University

Cooperative Institutions

Guangxi Academy of Agricultural Science

Chinese Academy of Agricultural Sciences

Ministry of Agriculture and Rural Affairs

Guangxi University

Sugarcane Research Center (Brazil)

State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources

G.B. Pant Institute of Himalayan Environment and Development

Graphic Era University

Shanghai Jiao Tong University

Govind Ballabh Pant University of Agriculture and Technology

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Physiological changes and transcriptome profiling in Saccharum spontaneum L. leaf under water stress and re-watering conditions

Scientific Reports (2021)

Changning Li Zhen Wang Qian Nong Li Lin Jinlan Xie

Abstract As the polyploidy progenitor of modern sugarcane, Saccharum spontaneum is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported on the mechanism of drought tolerance in S. spontaneum . Herein, the physiological changes of S. spontaneum GXS87-16 at three water-deficit levels (mild, moderate, and severe) and after re-watering during the elongation stage were investigated. RNA sequencing was utilized for global transcriptome profiling of GXS87-16 under severe drought and re-watered conditions. There were significant alterations in the physiological parameters of GXS87-16 in response to drought stress and then recovered differently after re-watering. A total of 1569 differentially expressed genes (DEGs) associated with water stress and re-watering were identified. Notably, the majority of the DEGs were induced by stress. GO functional annotations and KEGG pathway analysis assigned the DEGs to 47 GO categories and 93 pathway categories. The pathway categories were involved in various processes, such as RNA transport, mRNA surveillance, plant hormone signal transduction, and plant-pathogen interaction. The reliability of the RNA-seq results was confirmed by qRT-PCR. This study shed light on the regulatory processes of drought tolerance in S. spontaneum and identifies useful genes for genetic improvement of drought tolerance in sugarcane.

RNA-Seq

KEGG

Drought Tolerance

10.1038/s41598-021-85072-1

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Comparative analysis of sugarcane root transcriptome in response to the plant growth-promoting Burkholderia anthina MYSP113

PLoS ONE (2020)

Mukesh Kumar Malviya Changning Li Manoj Kumar Solanki Rajesh Kumar Singh Reemon Htun

The diazotrophic Burkholderia anthina MYSP113 is a vital plant growth-promoting bacteria and sugarcane root association. The present study based on a detailed analysis of sugarcane root transcriptome by using the HiSeq-Illumina platform in response to the strain MYSP113. The bacterium was initially isolated from the rhizosphere of sugarcane. To better understand biological, cellular, and molecular mechanisms, a de novo transcriptomic assembly of sugarcane root was performed. HiSeq-Illumina platformwas employed for the sequencing of an overall of 16 libraries at a 2×100 bp configuration. Differentially expressed genes (DEGs) analysis identified altered gene expression in 370 genes (total of 199 up-regulated genes and 171 down-regulated genes). Deciphering the huge datasets, concerning the functioning and production of biological systems, a high throughput genome sequencing analysis was attempted with Gene ontology functional analyses and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The report revealed a total of 148930 unigenes. 70414 (47.28%) of them were annotated successfully to Gene Ontology (GO) terms. 774 at 45 days, 4985 of 30 days and 15 days of 6846 terms were significantly regulated. GO analysis revealed that many genes involved in the metabolic, oxidation-reduction process and biological regulatory processes in response to strain MYSP113 and significantly enriched as compare to the control. Moreover, KEGG enriched results show that differentially expressed genes were classified into different pathway categories involved in various processes, such as nitrogen metabolism, plant hormone signal transduction, etc. The sample correlation analyses could help examine the similarity at the gene expression level. The reliability of the observed differential gene expression patterns was validated with quantitative real-time PCR (qRT-PCR). Additionally, plant enzymes activities such as peroxidase and superoxide dismutase were significantly increased in plant roots after the inoculation of strain MYSP113. The results of the research may help in understanding the plant growth-promoting rhizobacteria and plant interaction.

KEGG

Illumina dye sequencing

10.1371/journal.pone.0231206

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Bacillus subtilis NRRL B-30408: A potential inoculant for crops grown under rainfed conditions in the mountains

Journal of soil science and plant nutrition (2012)

Mukesh Kumar Malviya Avinash Sharma Ashok Pandey K. Rinu Priyanka Sati

On-farm inoculation trials have been conducted to evaluate the potential of Bacillus subtilis NRRL B-30408 in respect of plant growth promotion, for two consecutive years, under rainfed conditions at a mountain location in the Indian Himalayan Region (IHR).For this, one cereal (Oryza sativa) and two millets (Eleusine coracana and Echinochloa frumentacea), were used as test crops.Observations were recorded in terms of plant growth, yield, chlorophyll content, and root colonization by various groups of microorganisms.Positive influence of bacterial inoculation on all the three crops, statistically significant in case of most parameters examined as compared to untreated controls, was recorded in both the years.There was a significant increase in chlorophyll concentration in leaves of inoculated plants.While the bacterial inoculation stimulated the free living bacterial and actinomycetes populations in the rhizosphere, the fungal population was somewhat suppressed; indicative of the antifungal property of the inoculant.The treatment also enhanced the colonization of arbuscular mycorrhizae (AM), and the endophytes associated with the roots.In conclusion, the plant growth promotion, recorded as a result of microbial inoculation, is attributed to the microbial shift in the native microflora of the rhizosphere.Based on the results of this field study, B. subtilis NRRL B-30408 can be proposed as an efficient bioinoculant for the hill crops, grown under rainfed conditions of the mountain ecosystem.The main aim of this study was on farm demonstration of the microbe based technology with peoples' participation, with a view of adoption of the technology by the farmers.

10.4067/s0718-95162012005000034

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Developing mathematical model for diurnal dynamics of photosynthesis in Saccharum officinarum responsive to different irrigation and silicon application

PeerJ (2020)

Krishan K. Verma Kai-Chao Wu Chhedi Lal Verma Dongmei Li Mukesh Kumar Malviya

In the dynamic era of climate change, agricultural farming systems are facing various unprecedented problems worldwide. Drought stress is one of the serious abiotic stresses that hinder the growth potential and crop productivity. Silicon (Si) can improve crop yield by enhancing the efficiency of inputs and reducing relevant losses. As a quasi-essential element and the 2nd most abundant element in the Earth’s crust, Si is utilized by plants and applied exogenously to combat drought stress and improve plant performance by increasing physiological, cellular and molecular responses. However, the physiological mechanisms that respond to water stress are still not well defined in Saccharum officinarum plants. To the best of our knowledge, the dynamics of photosynthesis responsive to different exogenous Si levels in Saccharum officinarum has not been reported to date. The current experiment was carried out to assess the protective role of Si in plant growth and photosynthetic responses in Saccharum officinarum under water stress conditions. Saccharum officinarum cv. ‘GT 42’ plants were subjected to drought stress conditions (80–75%, 55–50% and 35–30% of soil moisture) after ten weeks of normal growth, followed by the soil irrigation of Si (0, 100, 300 and 500 mg L −1 ) for 8 weeks. The results indicated that Si addition mitigated the inhibition in Saccharum officinarum growth and photosynthesis, and improved biomass accumulation during water stress. The photosynthetic responses (photosynthesis, transpiration and stomatal conductance) were found down-regulated under water stress, and it was significantly enhanced by Si application. No phytotoxic effects were monitored even at excess (500 mg L −1 ). Soil irrigation of 300 mg L −1 of Si was more effective as 100 and 500 mg L −1 under water stress condition. It is concluded that the stress in Saccharum officinarum plants applied with Si was alleviated by improving plant fitness, photosynthetic capacity and biomass accumulation as compared with the control. Thus, this study offers new information towards the assessment of growth, biomass accumulation and physiological changes related to water stress with Si application in plants.

Saccharum officinarum

Stomatal Conductance

Water Use Efficiency

Saccharum

Photosynthetic efficiency

10.7717/peerj.10154

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Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.)

Scientific Reports (2017)

Changning Li Qian Nong Jinlan Xie Zeping Wang Qiang Liang

In plants, both abscisic acid (ABA) dependent and independent pathways form the basis for the response to environmental stresses. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) plays a central role in plant stress signal transduction. However, complete annotation and specific expression patterns of SnRK2s in sugarcane remain unclear. For the present study, we performed a full-length cDNA library survey of sugarcane, thus identifying ten SoSnRK2 genes via phylogenetic, local BLAST methods, and various bioinformatics analyses. Phylogenetic analysis indicated division of SoSnRK2 genes into three subgroups, similar to other plant species. Gene structure comparison with Arabidopsis suggested a unique evolutionary imprint of the SnRK2 gene family in sugarcane. Both sequence alignment and structural annotation provided an overview of the conserved N-terminal and variations of the C-terminal, suggesting functional divergence. Transcript and transient expression assays revealed SoSnRK2s to be involved in the responses to diverse stress signals, and strong ABA induction of SoSnRK2s in subgroup III. Co-expression network analyses indicated the existence of both conserved and variable biological functions among different SoSnRK2s members. In summary, this comprehensive analysis will facilitate further studies of the SoSnRK2 family and provide useful information for the functional validation of SoSnRK2s.

Multiple sequence alignment

10.1038/s41598-017-16152-4

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Citations (26)