Jian Cao

Sixth Affiliated Hospital of Kunming Medical University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Wei Sun

Central South University

Wanjia Zhang

First Affiliated Hospital of Hunan University of Traditional Chinese Medicine

Zhiyong Gao

Central South University

Zhitao Feng

University of California, Davis

Xin Jin

Lanzhou Jiaotong University

Renji Zheng

Central South University

Shihong Xu

University of Alberta

Edward A. Dennis

University of California, San Diego

Yiping Tan

Central South University

Wei Sun

Hainan Normal University

Cooperative Institutions

Central South University

Chinese Academy of Sciences

University of California, San Diego

University of Chinese Academy of Sciences

Education Department of Hunan Province

China University of Mining and Technology

Nanjing University

Sichuan University

Huazhong University of Science and Technology

Huaiyin Normal University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Assessing Phospholipase A2 Activity toward Cardiolipin by Mass Spectrometry

PLoS ONE (2013)

Yuan‐Hao Hsu Darren S. Dumlao Jian Cao Edward A. Dennis

Cardiolipin, a major component of mitochondria, is critical for mitochondrial functioning including the regulation of cytochrome c release during apoptosis and proper electron transport. Mitochondrial cardiolipin with its unique bulky amphipathic structure is a potential substrate for phospholipase A2 (PLA2) in vivo. We have developed mass spectrometric methodology for analyzing PLA2 activity toward various cardiolipin forms and demonstrate that cardiolipin is a substrate for sPLA2, cPLA2 and iPLA2, but not for Lp-PLA2. Our results also show that none of these PLA2s have significant PLA1 activities toward dilyso-cardiolipin. To understand the mechanism of cardiolipin hydrolysis by PLA2, we also quantified the release of monolyso-cardiolipin and dilyso-cardiolipin in the PLA2 assays. The sPLA2s caused an accumulation of dilyso-cardiolipin, in contrast to iPLA2 which caused an accumulation of monolyso-cardiolipin. Moreover, cardiolipin inhibits iPLA2 and cPLA2, and activates sPLA2 at low mol fractions in mixed micelles of Triton X-100 with the substrate 1-palmitoyl-2-arachidonyl-sn-phosphtidylcholine. Thus, cardiolipin functions as both a substrate and a regulator of PLA2 activity and the ability to assay the various forms of PLA2 is important in understanding its function.

Cardiolipins

10.1371/journal.pone.0059267

Cite

Citations (57)

Synthesis of selective heteroatomic collectors for the improved separation of sulfide minerals

Separation and Purification Technology (2022)

Wanjia Zhang Jian Cao Sihui Wu Wei Sun Zhitao Feng

HOMO/LUMO

Xanthate

Imidazole

10.1016/j.seppur.2022.120563

Cite

Citations (22)

Efficient heterogeneous photodegradation of Eosin Y by oxidized pyrite using the photo-Fenton process

Minerals Engineering (2022)

Wanjia Zhang Xu Jiang John Ralston Jian Cao Xin Jin

Eosin

Photodegradation

10.1016/j.mineng.2022.107972

Cite

Citations (12)

Utilization of phosphorylated starch as a selective depressant for serpentine in the flotation of nickel sulfide ore

Minerals Engineering (2024)

Chenxu Zhang Yiping Tan Fengxiang Yin Jian Zhao Zhiyong Gao

Depressant

Nickel sulfide

10.1016/j.mineng.2024.108906

Cite

Citations (3)

Different Aryl Thiourea Compounds as Flotation Collectors in a Lead–Zinc Sulfide Mixed System

Minerals (2023)

Bowen Li Wei Sun Zhiyong Gao Renji Zheng Jian Cao

The primary coexisting mineral with galena is sphalerite. Hence, it is critical to selectively separate galena from sphalerite by flotation. In this work, thiourea and related derivatives as potential flotation collectors for separating galena from sphalerite were investigated. Thiourea and its related derivatives were found to be effective selective collectors in batch flotation studies of a single mineral, with 1,1-diphenylthiourea (11DTA) emerging as the best choice. Galena has superior floatability compared to sphalerite in the presence of 11DTA, and the recovery difference between the two minerals at pH 8 (where the 11DTA concentration is 5 × 10−6 mol/L) is around 38%. This was revealed in batch flotation studies using artificial mixed minerals. Moreover, the findings from the measurements of adsorption amount, FTIR, zeta potential and XPS revealed that 11DTA has a strong adsorption on galena yet a relatively weak adsorption on sphalerite. Additionally, DFT calculations demonstrated that sphalerite exhibits stronger hydrophilicity than galena, and 11DTA possessed a better affinity for galena.

Sulfide Minerals

Lead sulfide

10.3390/min13060759

Cite

Citations (2)

Improved flotation of molybdenite from talc using a selective reagent scheme

Minerals Engineering (2021)

Wanjia Zhang Li‐Ming Tao Luobing Xun Zhongxu Qi Stephen Pooley

Molybdenite

Talc

Depressant

Froth flotation

Xanthate

Beneficiation

Gangue

10.1016/j.mineng.2021.107324

Cite

Citations (19)

Lipoprotein-associated phospholipase A ₂ : A paradigm for allosteric regulation by membranes

Proceedings of the National Academy of Sciences (2022)

Varnavas D. Mouchlis Daiki Hayashi Alexis M. Vasquez Jian Cao J. Andrew McCammon

Significance Lp-PLA 2 is a physiologically important human enzyme and an inflammatory biomarker for assessing risk factors associated with cardiovascular diseases. It is associated with low- and high-density lipoproteins in human plasma and acts on the outside of the phospholipid monolayer that coats these particles, in stark contrast to traditional PLA 2 enzymes that act on bilayer membranes. This study addresses the allosteric activation of Lp-PLA 2 by phospholipid monolayers and membranes, its precise selectivity and specificity for particular oxidized and short acyl-chain phospholipid substrates not previously possible. Of particular importance, this work identifies and confirms by site-directed mutagenesis a phospholipid head-group binding pocket distinct from known drug inhibitor binding pockets that informs us about Lp-PLA 2 ’s mechanism of action and creates opportunities for additional therapeutic approaches.

10.1073/pnas.2102953118

Cite

Citations (21)

Utilization of crown ether as selective collector for the flotation separation of pollucite from feldspar and quartz

Minerals Engineering (2024)

Yiping Tan Shuangke Li Xiaoping Xu Pan Chen Zhiyong Gao

10.1016/j.mineng.2024.109085

Cite

Citations (0)

Improved flotation separation of sulfide minerals by synthesized surfactant based on para-position methyl effect

Separation and Purification Technology (2022)

Wanjia Zhang Yunxia Wu Ruihua Fan Jian Cao Stephen Pooley