Inga Mohrbeck

Senckenberg am Meer

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Pedro Martínez Arbizu

Senckenberg am Meer

Terue C. Kihara

Senckenberg am Meer

Thomas Knebelsberger

Jade University of Applied Sciences

Adrian G. Glover

German Oceanographic Museum

Thomas G. Dahlgren

University of Gothenburg

Silke Laakmann

Helmholtz Institute for Functional Marine Biodiversity

Michael J. Raupach

Bavarian State Collection of Zoology

Helena Wiklund

University of Gothenburg

Annika Janssen

Senckenberg am Meer

Stefanie Kaiser

Senckenberg Research Institute and Natural History Museum Frankfurt/M

Cooperative Institutions

Senckenberg am Meer

Carl von Ossietzky Universität Oldenburg

Senckenberg Research Institute and Natural History Museum Frankfurt/M

Universität Hamburg

University of Bergen

Senckenberg Society for Nature Research

University of Hawaiʻi at Mānoa

Alfred-Wegener-Institut Helmholtz-Zentrum für Polar- und Meeresforschung

University of Łódź

Japan Agency for Marine-Earth Science and Technology

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Supplementary Video: An End-to-End DNA Taxonomy Methodology for Benthic Biodiversity Survey in the Clarion-Clipperton Zone, Central Pacific Abyss

Journal of Marine Science and Engineering (2015)

Adrian G. Glover Thomas G. Dahlgren Craig R. Smith Helena Wiklund Inga Mohrbeck

CLARION

End-to-end principle

10.5281/zenodo.35757

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Citations (0)

Species boundaries and phylogeographic patterns in new species ofNannoniscus(Janiroidea: Nannoniscidae) from the equatorial Pacific nodule province inferred from mtDNA and morphology

Zoological Journal of the Linnean Society (2020)

Stefanie Kaiser Terue C. Kihara Saskia Brix Inga Mohrbeck Annika Janssen

Abstract Spatial patterns of genetic variation (based on COI and 16S mtDNA) for morphologically similar species in the isopod genus Nannoniscus G.O. Sars. 1870 were examined that occur broadly across the Clarion Clipperton Fracture Zone (CCZ). Samples were obtained from five different licence areas as well as an Area of Particular Environmental Interest (APEI-6) with sites located at various distances (a few to several hundred kilometres) from one another. Applying three different species delimitation (SD) methods (sGMYC, mPTP and ABGD) of the molecular data, we could distinguish between four and 12 different molecular taxonomic operational units (MOTUs). Morphological analyses could confirm five distinct phenotypic clades that represent species new to science and are described here: Nannoniscus brenkei sp. nov., Nannoniscus hilario sp. nov., Nannoniscus magdae sp. nov., Nannoniscus menoti sp. nov. and Nannoniscus pedro sp. nov. Despite the assumed limited dispersal capacity of Nannoniscus species, we found haplotypes of two species to be geographically widespread (up to > 1400 km apart), as opposed to several divergent clades occurring in close vicinity or even sympatry. Geographic distance appeared to explain the phylogeographic structure of Nannoniscus species to some extent, although oceanographic features and level of environmental heterogeneity were probably equally important.

10.1093/zoolinnean/zlaa174

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Citations (49)

Tantulocarida (Crustacea) from the Southern Ocean deep sea, and the description of three new species of Tantulacus Huys, Andersen & Kristensen, 1992

Systematic Parasitology (2010)

Inga Mohrbeck Pedro Martínez Arbizu Thomas Glatzel

Synapomorphy

Animal ecology

Meiobenthos

10.1007/s11230-010-9260-0

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Citations (20)

The Application of DNA Barcodes for the Identification of Marine Crustaceans from the North Sea and Adjacent Regions

PLoS ONE (2015)

Michael J. Raupach Andrea Barco Dirk Steinke Jan Beermann Silke Laakmann

During the last years DNA barcoding has become a popular method of choice for molecular specimen identification. Here we present a comprehensive DNA barcode library of various crustacean taxa found in the North Sea, one of the most extensively studied marine regions of the world. Our data set includes 1,332 barcodes covering 205 species, including taxa of the Amphipoda, Copepoda, Decapoda, Isopoda, Thecostraca, and others. This dataset represents the most extensive DNA barcode library of the Crustacea in terms of species number to date. By using the Barcode of Life Data Systems (BOLD), unique BINs were identified for 198 (96.6%) of the analyzed species. Six species were characterized by two BINs (2.9%), and three BINs were found for the amphipod species Gammarus salinus Spooner, 1947 (0.4%). Intraspecific distances with values higher than 2.2% were revealed for 13 species (6.3%). Exceptionally high distances of up to 14.87% between two distinct but monophyletic clusters were found for the parasitic copepod Caligus elongatus Nordmann, 1832, supporting the results of previous studies that indicated the existence of an overlooked sea louse species. In contrast to these high distances, haplotype-sharing was observed for two decapod spider crab species, Macropodia parva Van Noort & Adema, 1985 and Macropodia rostrata (Linnaeus, 1761), underlining the need for a taxonomic revision of both species. Summarizing the results, our study confirms the application of DNA barcodes as highly effective identification system for the analyzed marine crustaceans of the North Sea and represents an important milestone for modern biodiversity assessment studies using barcode sequences.

DNA Barcoding

Isopoda

Barcode

Monophyly

Gammarus

10.1371/journal.pone.0139421

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Citations (139)

High-Throughput Sequencing—The Key to Rapid Biodiversity Assessment of Marine Metazoa?

PLoS ONE (2015)

Inga Mohrbeck Michael J. Raupach Pedro Martínez Arbizu Thomas Knebelsberger Silke Laakmann

The applications of traditional morphological and molecular methods for species identification are greatly restricted by processing speed and on a regional or greater scale are generally considered unfeasible. In this context, high-throughput sequencing, or metagenetics, has been proposed as an efficient tool to document biodiversity. Here we evaluated the effectiveness of 454 pyrosequencing in marine metazoan community analysis using the 18S rDNA: V1-V2 region. Multiplex pyrosequencing of the V1-V2 region was used to analyze two pooled samples of DNA, one comprising 118 and the other 37 morphologically identified species, and one natural sample taken directly from a North Sea zooplankton community. A DNA reference library comprising all species represented in the pooled samples was created by Sanger sequencing, and this was then used to determine the optimal similarity threshold for species delineation. The optimal threshold was found at 99% species similarity, with 85% identification success. Pyrosequencing was able to identify between fewer species: 67% and 78% of the species in the two pooled samples. Also, a large number of sequences for three species that were not included in the pooled samples were amplified by pyrosequencing, suggesting preferential amplification of some genotypes and the sensitivity of this approach to even low levels of contamination. Conversely, metagenetic analysis of the natural zooplankton sample identified many more species (particularly gelatinous zooplankton and meroplankton) than morphological analysis of a formalin-fixed sample from the same sampling site, suggesting an increased level of taxonomic resolution with pyrosequencing. The study demonstrated that, based on the V1-V2 region, 454 sequencing does not provide accurate species differentiation and reliable taxonomic classification, as it is required in most biodiversity monitoring. The analysis of artificially prepared samples indicated that species detection in pyrosequencing datasets is complicated by potential PCR-based biases and that the V1-V2 marker is poorly resolved for some taxa.

Pyrosequencing

Sanger sequencing

Multiplex

Environmental DNA

DNA Barcoding

10.1371/journal.pone.0140342

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Citations (55)

An End-to-End DNA Taxonomy Methodology for Benthic Biodiversity Survey in the Clarion-Clipperton Zone, Central Pacific Abyss

Journal of Marine Science and Engineering (2015)

Adrian G. Glover Thomas G. Dahlgren Helena Wiklund Inga Mohrbeck Craig R. Smith

Recent years have seen increased survey and sampling expeditions to the Clarion-Clipperton Zone (CCZ), central Pacific Ocean abyss, driven by commercial interests from contractors in the potential extraction of polymetallic nodules in the region. Part of the International Seabed Authority (ISA) regulatory requirements are that these contractors undertake environmental research expeditions to their CCZ exploration claims following guidelines approved by the ISA Legal and Technical Commission (ISA, 2010). Section 9 (e) of these guidelines instructs contractors to “…collect data on the sea floor communities specifically relating to megafauna, macrofauna, meiofauna, microfauna, nodule fauna and demersal scavengers”. There are a number of methodological challenges to this, including the water depth (4000–5000 m), extremely warm surface waters (~28 °C) compared to bottom water (~1.5 °C) and great distances to ports requiring a large and long seagoing expedition with only a limited number of scientists. Both scientists and regulators have recently realized that a major gap in our knowledge of the region is the fundamental taxonomy of the animals that live there; this is essential to inform our knowledge of the biogeography, natural history and ultimately our stewardship of the region. Recognising this, the ISA is currently sponsoring a series of taxonomic workshops on the CCZ fauna and to assist in this process we present here a series of methodological pipelines for DNA taxonomy (incorporating both molecular and morphological data) of the macrofauna and megafauna from the CCZ benthic habitat in the recent ABYSSLINE cruise program to the UK-1 exploration claim. A major problem on recent CCZ cruises has been the collection of high-quality samples suitable for both morphology and DNA taxonomy, coupled with a workflow that ensures these data are made available. The DNA sequencing techniques themselves are relatively standard, once good samples have been obtained. The key to quality taxonomic work on macrofaunal animals from the tropical abyss is careful extraction of the animals (in cold, filtered seawater), microscopic observation and preservation of live specimens, from a variety of sampling devices by experienced zoologists at sea. Essential to the long-term iterative building of taxonomic knowledge from the CCZ is an “end-to-end” methodology to the taxonomic science that takes into account careful sampling design, at-sea taxonomic identification and fixation, post-cruise laboratory work with both DNA and morphology and finally a careful sample and data management pipeline that results in specimens and data in accessible open museum collections and online repositories.

10.3390/jmse4010002

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Citations (84)

Description of Pseudeuchaeta vulgaris n. sp. (Copepoda: Calanoida), a new aetideid species from the deep Pacific Ocean with notes on the biogeography of benthopelagic aetideid calanoids

Marine Biodiversity (2016)

Elena L. Markhaseva Inga Mohrbeck Jasmin Renz

Calanoida

10.1007/s12526-016-0527-9

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Citations (11)

Diversity, distribution and composition of abyssal benthic Isopoda in a region proposed for deep-seafloor mining of polymetallic nodules: a synthesis

Marine Biodiversity (2023)

Stefanie Kaiser Magdalini Christodoulou Annika Janssen Terue C. Kihara Inga Mohrbeck

Abstract Due to the increasing challenge of meeting human demands for metals from land-based resources, interest in extracting mineral ores from the deep sea has gained momentum in recent years. Targeted mining of deep-seabed minerals could have adverse effects on the associated ecosystems, but knowledge on the biological communities found there, their structure and functions is still limited. The focus of this study is to provide an overview on isopod crustaceans from the Clarion Clipperton Fracture Zone (CCFZ), an area well-known for its abundance of high-grade polymetallic nodules. Isopods generally comprise an important part of the macrofaunal communities of soft deep-sea sediments and indeed are one of the most dominant macrobenthic groups in the CCFZ. In this review, we have compiled all available data and information on isopod diversity and distribution in the CCFZ in a hybrid manner, which includes published data from the literature as well as the analysis of previously unpublished sources and newly collected data. Although isopods are one of the more prevalent and better-known groups of the CCFZ fauna, this study shows that it is still remarkably difficult to obtain a clear perception of isopod diversity and distribution, as well as the factors that could be responsible for the observed patterns. In many places, knowledge remains incomplete, which is largely due to the low sampling and taxonomic effort, non-standardised sample protocols and the limited taxonomic inter-calibration between studies. The latter is pivotal due to the high proportion of undescribed and presumably new species that typically occur there. An important starting point would therefore be to increase sampling effort and its spatial and temporal coverage in a standardised way, to intensify (integrative) taxonomic work as well as to facilitate sample and data exchange between scientists and contractors. These are fundamental requirements to improve our understanding of the biodiversity of isopods, but also of other faunal groups, in the CCFZ, before mining operations begin.

Isopoda

Abyssal plain

Abyssal zone

10.1007/s12526-023-01335-2

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Citations (3)

DNA barcoding and cryptic diversity of deep-sea scavenging amphipods in the Clarion-Clipperton Zone (Eastern Equatorial Pacific)

Marine Biodiversity (2021)

Inga Mohrbeck Tammy Horton Anna Jażdżewska Pedro Martínez Arbizu

Abstract The Clarion-Clipperton Zone (CCZ), located in the abyssal equatorial Pacific, has been subject to intensive international exploration for polymetallic nodule mining over the last four decades. Many studies have investigated the potential effects of mining on deep-sea ecosystems and highlighted the importance of defining environmental baseline conditions occurring at potential mining sites. However, current information on biodiversity and species distributions in the CCZ is still scarce and hampers the ability to effectively manage and reduce the potential impacts of mining activities. As part of the regulatory regimes adopted by the International Seabed Authority, concession holders are required to conduct an environmental impact assessment and gather baseline data on biodiversity and community structure in relation to their license areas. In the present study, we used an integrative molecular and morphological approach to assess species richness and genetic variation of deep-sea scavenging amphipods collected in two nodule-mining exploration areas (UK-1 and OMS-1 areas) and one Area of Particular Environmental Interest (APEI-6) in the eastern part of the CCZ. We analyzed the DNA sequences of the cytochrome c oxidase subunit I gene of 645 specimens belonging to ten distinct morphospecies. Molecular data uncover potential cryptic diversity in two investigated species, morphologically identified as Paralicella caperesca Shulenberger & Barnard, 1976 and Valettietta cf. anacantha (Birstein & Vinogradov, 1963). Our study highlights the importance of using molecular tools in conjunction with traditional morphological methods for modern biodiversity assessment studies, particularly to evaluate morphologically similar individuals and incomplete specimens. The results of this study can help determine species identity and ranges, information which can feed into environmental management.

DNA Barcoding

Species complex

Abyssal zone

Marine ecosystem

10.1007/s12526-021-01170-3

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Citations (20)