Marta Prado

Universidade de Santiago de Compostela

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Alejandro Garrido‐Maestu

International Iberian Nanotechnology Laboratory

Laura Muinelo‐Romay

Instituto de Investigación Sanitaria de Santiago

Joana Carvalho

Universidade de Santiago de Compostela

Alexandra Teixeira

Administração Regional de Saúde de Lisboa e Vale do Tejo

Luís Lima

Instituto Português de Oncologia Francisco Gentil

Verónica Gros

Ministerio de Transportes, Movilidad y Agenda Urbana

Manuel Aira

Universidade de Vigo

Jorge Barros‐Velázquez

Universidade de Santiago de Compostela

R. J. De Miguel

University of Córdoba

Maruxa Abalo

Universidade de Santiago de Compostela

Cooperative Institutions

International Iberian Nanotechnology Laboratory

Universidade de Santiago de Compostela

Instituto de Investigacións Mariñas

Universidade do Porto

University of Minho

Universidade de Vigo

Instituto de Investigaciones Biomédicas Sols-Morreale

University of Teramo

Clinical Academic Center of Braga

Instituto Português de Oncologia Francisco Gentil

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Profiling DNA mutation patterns by SERS fingerprinting for supervised cancer classification

Biosensors and Bioelectronics (2020)

Lei Wu Alexandra Teixeira Alejandro Garrido‐Maestu Laura Muinelo‐Romay Luís Lima

Multiplex

Biochip

Surface-Enhanced Raman Spectroscopy

Personalized Medicine

Profiling (computer programming)

DNA profiling

10.1016/j.bios.2020.112392

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Citations (44)

Earthworm activity reduces bacterial pathogen loads in sewage sludge

Environmental Science and Pollution Research (2024)

Manuel Aira Alejandro Garrido‐Maestu Marta Prado Jorge Domínguez

Sewage sludge

Biosolids

Oligochaeta (plant)

10.1007/s11356-024-35358-4

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Faster monitoring of the invasive alien species (IAS) Dreissena polymorpha in river basins through isothermal amplification

Scientific Reports (2021)

Joana Carvalho Alejandro Garrido‐Maestu Sarah Azinheiro Pablo Fuciños Jorge Barros‐Velázquez

Abstract Zebra mussel ( Dreissena polymorpha ) is considered as one of the 100 most harmful IAS in the world. Traditional detection methods have limitations, and PCR based environmental DNA detection has provided interesting results for early warning. However, in the last years, the development of isothermal amplification methods has received increasing attention. Among them, loop-mediated isothermal amplification (LAMP) has several advantages, including its higher tolerance to the presence of inhibitors and the possibility of naked-eye detection, which enables and simplifies its potential use in decentralized settings. In the current study, a real-time LAMP (qLAMP) method for the detection of Dreissena polymorpha was developed and tested with samples from the Guadalquivir River basin, together with two real-time PCR (qPCR) methods using different detection chemistries, targeting a specific region of the mitochondrial gene cytochrome C oxidase subunit I. All three developed approaches were evaluated regarding specificity, sensitivity and time required for detection. Regarding sensitivity, both qPCR approaches were more sensitive than qLAMP by one order of magnitude, however the qLAMP method proved to be as specific and much faster being performed in just 9 min versus 23 and 29 min for the qPCR methods based on hydrolysis probe and intercalating dye respectively.

Dreissena

10.1038/s41598-021-89574-w

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Citations (16)

Surface monitoring of L. monocytogenes by real-time fluorescence and colorimetric LAMP

Applied Microbiology and Biotechnology (2024)

Maruxa Abalo Alexandre Lamas Carla Teixeira Marta Prado Alejandro Garrido‐Maestu

Listeria monocytogenes is a major foodborne pathogen affecting developing, and developed countries. The analysis of food contact surfaces in food industries is key for better controlling this pathogen. The current study focused on the development, optimization, and evaluation of a rapid and simple method for the detection of L. monocytogenes on stainless steel surfaces, suitable for decentralized setups, taking advantage of Loop-mediated isothermal amplification (LAMP). This was accomplished using a general pre-enrichment broth (TSB), with a simple DNA extraction based on a chelating resin, and final isothermal amplification. Two different detection strategies were tested, real-time fluorescence and naked-eye colorimetric, which were evaluated after 5, 7, and 24 h of pre-enrichment. Regardless the detection chemistry selected, after 5-7 h of pre-enrichment, 10

Naked eye

Isothermal process

10.1007/s00253-024-13318-9

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