Dan Hu

Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University

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Liandong Zhu

Wuhan University

Shuangxi Li

Zhejiang Normal University

Zhihong Yin

South China University of Technology

Chenchen Liu

Shandong Mental Health Center

Guo‐Dong Chen

Jinan University

Ruoyu Chu

Wuhan University

Hao Gao

Jinan University

Xin‐Sheng Yao

Shenyang Pharmaceutical University

Zhiping Tian

Institute of Atmospheric Physics

Dabang Jiang

Institute of Atmospheric Physics

Cooperative Institutions

Chinese Academy of Sciences

Jinan University

University of Chinese Academy of Sciences

Wuhan University

Hubei University of Technology

Nanjing General Hospital of Nanjing Military Command

Research Center for Eco-Environmental Sciences

Zhejiang University

Ministry of Natural Resources

Huadong Hospital

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Functional and structural dissection of glycosyltransferases underlying the glycodiversity of wolfberry-derived bioactive ingredients lycibarbarspermidines

Nature Communications (2024)

Shao-Yang Li Gao‐Qian Wang Long Liang Jia-Ling Gao Zheng-Qun Zhou

Abstract Lycibarbarspermidines are unusual phenolamide glycosides characterized by a dicaffeoylspermidine core with multiple glycosyl substitutions, and serve as a major class of bioactive ingredients in the wolfberry. So far, little is known about the enzymatic basis of the glycosylation of phenolamides including dicaffeoylspermidine. Here, we identify five lycibarbarspermidine glycosyltransferases, LbUGT1-5, which are the first phenolamide-type glycosyltransferases and catalyze regioselective glycosylation of dicaffeoylspermidines to form structurally diverse lycibarbarspermidines in wolfberry. Notably, LbUGT3 acts as a distinctive enzyme that catalyzes a tandem sugar transfer to the ortho-dihydroxy group on the caffeoyl moiety to form the unusual ortho-diglucosylated product, while LbUGT1 accurately discriminates caffeoyl and dihydrocaffeoyl groups to catalyze a site-selective sugar transfer. Crystal structure analysis of the complexes of LbUGT1 and LbUGT3 with UDP, combined with molecular dynamics simulations, revealed the structural basis of the difference in glycosylation selectivity between LbUGT1 and LbUGT3. Site-directed mutagenesis illuminates a conserved tyrosine residue (Y389 in LbUGT1 and Y390 in LbUGT3) in PSPG box that plays a crucial role in regulating the regioselectivity of LbUGT1 and LbUGT3. Our study thus sheds light on the enzymatic underpinnings of the chemical diversity of lycibarbarspermidines in wolfberry, and expands the repertoire of glycosyltransferases in nature.

10.1038/s41467-024-49010-9

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Citations (4)

Nutrient removal and lipid production by the co-cultivation of Chlorella vulgaris and Scenedesmus dimorphus in landfill leachate diluted with recycled harvesting water

Bioresource Technology (2022)

Chunming Tang Xinxin Gao Dan Hu Dian Dai Mingxiang Qv

Chlorella vulgaris

Scenedesmus

10.1016/j.biortech.2022.128496

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Citations (16)

Input, stocks and output flows of urban residential building system in Beijing city, China from 1949 to 2008

Resources Conservation and Recycling (2010)

Dan Hu Fang You Yanhua Zhao Ye Yuan Tianxing Liu

Building material

Urban construction

10.1016/j.resconrec.2010.03.011

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Citations (146)

An integrated material metabolism model for stocks of urban road system in Beijing, China

The Science of The Total Environment (2013)

Zhen Guo Dan Hu Fuhua Zhang Guolong Huang Qiang Xiao

Stock (firearms)

10.1016/j.scitotenv.2013.10.041

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Citations (70)

Isolation and characterization of a native avirulent strain of Streptococcus suis serotype 2: a perspective for vaccine development

Scientific Reports (2015)

Xinyue Yao Ming Li Jing Wang Changjun Wang Dan Hu

Abstract Streptococcus suis , an emerging infectious pathogen, is the cause of two large-scale outbreaks of human streptococcal toxic shock syndrome in China and has attracted much attention from the scientific community. The genetic basis of its pathogenesis remains enigmatic and no effective prevention measures have been established. To better understand the virulence differentiation of S. suis and develop a promising vaccine, we isolated and sequenced a native avirulent S. suis strain (05HAS68). Animal experiments revealed that 05HAS68 is an avirulent strain and could protect piglets from the attack of virulent strains. Comparative genomics analyses demonstrated the genetic basis for the lack of virulence in 05HAS68, which is characterized by the absence of some important virulence-associated factors and the intact 89K pathogenicity island. Lack of virulence was also illustrated by reduced survival of 05HAS68 compared to a virulent strain in pig whole blood. Further investigations revealed a large-scale genomic rearrangement in 05HAS68, which was proposed to be mediated by transposase genes and/or prophages. This genomic rearrangement may have caused the genomic diversity of S. suis and resulted in biological discrepancies between 05HAS68 and highly virulent S. suis strains.

Streptococcus suis

Isolation

Strain (injury)

10.1038/srep09835

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Citations (22)

Dynamic characteristics of a water resource structure in an urban ecological system: structure modelling based on input–occupancy–output technology

Journal of Cleaner Production (2016)

Qiang Xiao Dan Hu

Water use

Occupancy

Consumption

10.1016/j.jclepro.2016.02.101

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Citations (11)

Limited Skill of Projected Land Precipitation by IPCC Models During 2002−2020

Journal of Geophysical Research Atmospheres (2023)

Dan Hu Zhiping Tian Xianmei Lang Dabang Jiang

Abstract Climate models are widely used to project future climate changes and analyze underlying mechanisms. However, it remains unknown whether the earlier precipitation projections match the subsequent observations in recent decades, which is the scientific basis for the confidence of precipitation projections and associated impacts. Here, we find models in the Third (TAR), Fourth (AR4), and Fifth (AR5) Assessment Reports of the Intergovernmental Panel on Climate Change (IPCC) skillfully project the subsequent climatological changes in global mean land precipitation for 2002–2020, 2008–2020, and 2014–2020 (relative to 1980–1999 climatology) from several to 10 years ahead, respectively. Skillful regional projections are mainly found in the northern mid to high latitudes. IPCC models are less skillful in projecting subsequent changes in land precipitation at regional scales than at global scales, which is likely to be at least partly explained by the lower signal‐to‐noise ratio.

10.1029/2022jd037851

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A new metallogenic model for the giant manganese deposits in northeastern Guizhou, China

Ore Geology Reviews (2022)

Qi Zhou Chonglong Wu Xiangyun Hu Bingnan Yang Xialin Zhang

The recently discovered multiple world-class Nanhuanian manganese (Mn) ore deposits at Datangpo of northeastern Guizhou province in southern China provides substantial amount of Mn that can be economically important for industrial usage. Studies that we previously conducted suggest that these Mn ore deposits are likely manifestations of the deposition of volatile-rich Mn-bearing ore-forming materials originating from deep reservoirs. However, ore-forming structures that transport ore-forming materials, if existed, have not yet been identified. Here, integrated with three-dimensional (3D) geological modeling, aeromagnetic △T data processing and Bouguer gravity anomaly analysis, we found that the intersections of the northwest (NW)-trending early rift basins and the near east–west (EW)-trending Nanhua rift basins overlap with the localities of the giant Mn ore deposits in northeastern Guizhou. In addition, a seismic reflection profiles passing through the Mn mining district reveals an upward-widen, funnel-shaped seismically abnormal structure with a dimension of about 3-km-wide and 45-km-deep. The seismically abnormal structure highly likely represents a deep-seated diapir channel beneath the mining district. Such a structure could contribute to the transportation of Mn-bearing materials that are responsible for the final deposition of Mn ore deposits. This study not only offers a visualization of a deep-seated channel that may be part of ore-guiding structures, but also sheds light on a new metallogenic model of Mn ore deposit.

Metallogeny

Mineral resource classification

Prospecting

10.1016/j.oregeorev.2022.105070

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Citations (6)

A continuous dynamic feature of the distribution of soil temperature and horizontal heat flux next to external walls in different orientations of construction sites in the autumn of Beijing, China

Journal of Cleaner Production (2015)

Hongxuan Zhou Yuanzheng Li Kaipeng Xu Hongxing Zhang Dan Hu

Urban Heat Island

Urban ecosystem

Ecotone

10.1016/j.jclepro.2015.10.120

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Citations (6)

Green biosynthesis of rare DHA-phospholipids by lipase-catalyzed transesterification with edible algal oil in solvent-free system and catalytic mechanism study

Frontiers in Bioengineering and Biotechnology (2023)

Tiantian Zhang Binglin Li Zhulin Wang Dan Hu Xiaoli Zhang

Docosahexaenoic acid (DHA)-enriched phosphatidylcholine (PC) has received significant scientific attention due to the health benefits in food and pharmaceutical products. In this work, the edible algal oil rich in DHA-triacylglycerol (DHA-TAG) without pretreatment was first used as the DHA donor for the transesterification of phospholipids (PLs) to prepare three kinds of rare PLs, including DHA-PC, DHA-phosphatidylethanolamine (DHA-PE), and DHA-phosphatidylserine (DHA-PS). Here, 153 protein structures of triacylglycerol lipase (EC 3.1.1.3) were virtually screened and evaluated by transesterification. PLA1 was the best candidate due to a higher DHA incorporation. Results showed that the transesterification of PC with DHA-TAG at 45°C and 0.7% water content (without additional water addition) could produce DHA-PC with 39.1% DHA incorporation at 30 min. The different DHA donors, including forms of fatty acid, methyl ester, and triglycerides, were compared. Molecular dynamics (MD) was used to illustrate the catalytic mechanism at the molecular level containing the diffusions of substrates, the structure-activity relationship of PLA1, and the effect of water content.

Phosphatidylethanolamine

10.3389/fbioe.2023.1158348

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Citations (4)