Ruicai Long

Chinese Academy of Agricultural Sciences

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Mingna Li

Chinese Academy of Agricultural Sciences

Junmei Kang

Chinese Academy of Agricultural Sciences

Yan Sun

National Clinical Research

Huiting Cui

Xiamen University

Shihao Cao

China Agricultural University

Qingchuan Yang

Chinese Academy of Agricultural Sciences

Kun Zhang

Fujian Agriculture and Forestry University

Yan Li

Xiamen University

Ding Wang

University of California, Los Angeles

Xianyang Li

Chinese Academy of Agricultural Sciences

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Chinese Academy of Agricultural Sciences

China Agricultural University

Institute of Animal Sciences

Chinese Academy of Medical Sciences & Peking Union Medical College

Southern Medical University

Peking Union Medical College Hospital

Ministry of Agriculture and Rural Affairs

Xiamen University

Huazhong Agricultural University

Beijing Forestry University

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Quantitative proteomic analysis using iTRAQ to identify salt-responsive proteins during the germination stage of two Medicago species

Scientific Reports (2018)

Ruicai Long Yanli Gao Hao Sun Tiejun Zhang Xiao Li

Salt stress is one of the primary abiotic stresses responsible for decreasing crop yields worldwide. Germinating seeds can be greatly influenced by saline conditions. In this study, the physiological and phenotypic changes induced by salt treatments (10-50 mM NaCl and Na2SO4 mixtures) were analysed for Zhongmu-3 (Medicago sativa) and R108 (Medicago truncatula) seedlings. Our observations indicated that Zhongmu-3 was more salt-tolerant than R108. To characterize the protein expression profiles of these two Medicago species in response to salt stress, an iTRAQ-based quantitative proteomic analysis was applied to examine salt-responsive proteins. We identified 254 differentially changed salt-responsive proteins. Compared with control levels, the abundance of 121 proteins increased and 44 proteins decreased in salt-treated Zhongmu-3 seedlings, while 119 proteins increased and 18 proteins decreased in R108 seedlings. Moreover, 48 differentially changed proteins were common to Zhongmu-3 and R108 seedlings. A subsequent functional annotation indicated these proteins influenced diverse processes, such as catalytic activity, binding, and antioxidant activity. Furthermore, the corresponding transcript levels of 15 differentially changed proteins were quantified by qRT-PCR. The data presented herein provide new insights into salt-responsive proteins, with potential implications for enhancing the salt tolerance of Medicago species.

Medicago sativa

Medicago

Medicago truncatula

10.1038/s41598-018-27935-8

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Citations (26)

Comprehensive analysis of epigenetic modifications in alfalfa under cadmium stress

Journal of Hazardous Materials (2024)

Lin Chen Xianyang Li Hao Liu Fei He Mingna Li

10.1016/j.jhazmat.2024.136545

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Citations (1)