AIM:To evaluate the efficacy of centralized culture and possible influencing factors. METHODS:From January 2010 to July 2012, 66452 patients with suspected Helicobacter pylori (H.pylori ) infection from 26 hospitals in Zhejiang and Jiangsu Provinces in China underwent gastrointestinal endoscopy.Gastric mucosal biopsies were taken from the antrum for culture.These biopsies were transported under natural environmental temperature to the central laboratory in Hangzhou city and divided into three groups based on their transport time: 5, 24 and 48 h.The culture results were reported after 72 h and the positive culture rates were analyzed by a χ 2 test.An additional 5736 biopsies from H. pylori -positive patients (5646 rapid urease test-positive and 9014 C-urease breath test-positive) were also cultured for quality control in the central laboratory setting. RESULTS:The positive culture rate was 31.66%(21036/66452) for the patient samples and 71.72% (4114/5736) for the H. pylori -positive quality control specimens.In the 5 h transport group, the positive culture rate was 30.99% (3865/12471), and 32.84% (14960/45553) in the 24 h transport group.In contrast, the positive culture rate declined significantly in the 48 h transport group (26.25%;P < 0.001).During transportation, the average natural temperature increased from 4.67 to 29.14 ℃, while the positive culture rate declined from 36.67% (1462/3987) to 24.12% (1799/7459).When the temperature exceeded 24 ℃, the positive culture rate decreased significantly, especially in the 48 h transport group (23.17%). CONCLUSION:Transportation of specimens within 24 h and below 24 ℃ is reasonable and acceptable for centralized culture of multicenter H. pylori samples.
To determine the prevalence of Helicobacter pylori infection and the antibiotic susceptibility of H. pylori in patients after partial gastrectomy.Patients who underwent gastroscopy from January 2009 to November 2017 and had a history of partial gastrectomy were retrospectively enrolled in the remnant stomach group. Contemporary non-gastrectomized patients with an endoscopic diagnosis of chronic gastritis were enrolled in the non-operated stomach group. The detection of H. pylori infection was performed by culture and histology. The in vitro antimicrobial susceptibility was examined by the agar dilution method on strains from gastric biopsies.In this study, a total of 728 gastrectomized and 5035 non-gastrectomized patients were included. There was a significantly lower prevalence of H. pylori infection in the gastric-remnant patients (8.65%) than in the non-gastrectomized patients (17.76%) (P < .001) with the diagnostic method of culture. In the gastric-remnant patients, the H. pylori strains had resistance rates to metronidazole, clarithromycin, levofloxacin, amoxicillin, and furazolidone of 100%, 20.63%, 22.22%, 0%, and 0%, respectively. In the nongastrectomized patients, H. pylori resistance to metronidazole, clarithromycin, levofloxacin, amoxicillin, and furazolidone was 90.49%, 24.61%, 21.70%, 0.22%, and 0.11%, respectively. Gastric-remnant patients had a significantly higher metronidazole resistance rate than non-gastrectomized patients (P = .005). Moreover, no significant changes in the resistance to 5 antibiotics were observed among the gastric-remnant patients from different age, gender, and surgical indication groups.Patients after partial gastrectomy showed a lower prevalence of H. pylori infection. Gastric-remnant patients were more likely to harbor metronidazole-resistant H. pylori strains.
'/%3e%3cuse xlink:href='%23a' transform='rotate(23.036 .093 25.536)'/%3e%3cuse xlink:href='%23a' transform='rotate(45.87 1.273 16.18)'/%3e%3cuse xlink:href='%23a' transform='rotate(69.945 .996 12.078)'/%3e%3cuse xlink:href='%23a' transform='rotate(20.66 -19.689 31.932)'/%3e%3c/svg%3e)